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Animal feeding stuffs: Methods of sampling and analysis. Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography
Description
Animal feeding stuffs: Methods of sampling and analysis. Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography1 Scope This document specifies a test method for the determination of theobromine in feed material or compound feed in the working range 27 mg kg to 307 mg kg using liquid chromatography coupled to a UV detector (HPLC UV) or in the working range 49 mg kg to 307 mg kg using liquid chromatography with tandem mass spectrometry (LC MS MS). This method has been fully validated using complementary compound feed for adult dogs and complementary compound
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